Coding

Part:BBa_K209090:Design

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-10-14)


ACA-linker-PI3K1(fl)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1408
    Illegal EcoRI site found at 7441
    Illegal XbaI site found at 3922
    Illegal SpeI site found at 4399
    Illegal SpeI site found at 5515
    Illegal PstI site found at 7589
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1408
    Illegal EcoRI site found at 7441
    Illegal SpeI site found at 4399
    Illegal SpeI site found at 5515
    Illegal PstI site found at 7589
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1408
    Illegal EcoRI site found at 7441
    Illegal BglII site found at 3145
    Illegal BglII site found at 4074
    Illegal BamHI site found at 8442
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1408
    Illegal EcoRI site found at 7441
    Illegal XbaI site found at 3922
    Illegal SpeI site found at 4399
    Illegal SpeI site found at 5515
    Illegal PstI site found at 7589
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1408
    Illegal EcoRI site found at 7441
    Illegal XbaI site found at 3922
    Illegal SpeI site found at 4399
    Illegal SpeI site found at 5515
    Illegal PstI site found at 7589
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Aar1 AB BC CD


Source

plasmid

References